Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles

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Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles.

BACKGROUND The aim of this study was to analyse the outcome of closed blastocyst vitrification of embryos biopsied at the cleavage stage. METHODS Vitrification of supernumerary blastocysts was performed using the closed CBS-VIT High Security straws. Warming cycles (n = 100) for patients with preimplantation genetic diagnosis (PGD) and/or aneuploidy screening in the fresh cycle were analysed. ...

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Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy.

BACKGROUND In order to optimize blastocyst cryopreservation, vitrification was introduced as the routine procedure instead of slow freezing. The outcome of a closed blastocyst vitrification system was evaluated in relation to the blastocyst score before cryopreservation in single embryo transfers (SETs). METHODS Supernumerary blastocysts of IVF/ICSI patients with a fresh Day 5 transfer were v...

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Cryopreservation of biopsied embryos at the blastocyst stage.

BACKGROUND The availability of an efficient cryopreservation program is especially important in the case of embryos that have undergone blastomere biopsy for PGD. Unfortunately, the freezing/thawing of biopsied embryos has given disappointing results when performed at the cleavage stage. In this study, embryos diagnosed as normal after PGD were grown to the blastocyst stage, frozen and thawed f...

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Reproductive outcomes of retransferring retained embryos in blastocyst transfer cycles

OBJECTIVE To determine the incidence of embryo retention (ER) in the transfer catheter following embryo transfer (ET) in blastocyst transfer and investigate whether retransferring retained embryos has an impact on reproductive outcomes in patients undergoing in vitro fertilization-ET. METHODS We retrospectively analyzed the records of 1,131 blastocyst transfers, which comprised 223 single bla...

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Cryopreservation of Day 8 equine embryos after blastocyst micromanipulation and vitrification.

Pregnancy rates after cryopreservation of large equine blastocyst stage embryos have remained lower than other domesticated livestock species. It is generally accepted that the embryonic capsule is the primary barrier to cryoprotectant entry into the embryo proper and techniques need to be developed to circumvent this obstacle. Therefore, the objective of this study was to develop an efficient ...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2010

ISSN: 0268-1161,1460-2350

DOI: 10.1093/humrep/deq338